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The Department of Pathology and Cell Biology at Columbia University Irving Medical Center seeks a highly qualified Staff Associate I who will perform a research support role for the following: they will help to manage the mouse colony, which will involve setting up breeding pairs, weaning pups and performing genotyping on mice and the associated record keeping that entails. They will perform basic tissue culture in a support role - changing media.They will perform immunohistochemistry on both cell cultures and on tissue sections and will also set up imaging sessions on a confocal microscope. Provided the aformentioned tasks are accomplished, the individual will have the opportunity to lead a research project of their own, which they can present in group meeting and at conferences.

At CUIMC, we stand together because diverse experiences, perspective, and values enrich every dimension of our work. Join our team and see how your unique skills and experiences can create a real impact by changing lives.

Job Function:

• Mouse husbandry: management of mouse colony, which entails detailed electronic record keeping, monitoring breeding pairs, weaning pups.
• Genotyping: obtaining small biological sample of each mouse, tagging their ear for record keeping purposes, digesting DNA, running PCR reaction, running gel, updating records for gel image and genotype for mice in colony.
• Tissue Culture: Basic tissue culture - maintenance of primary neuronal cultures, preparation of stock TC reagents such as media, aliquoting supplements.
• Immunohistochemistry: Tissue preparation (sectioning), performing immunohistochemical steps on tissue, mounting and coverslipping.
• Imaging: Setting up confocal imaging sessions for tissue and cells where IHC has been performed. Booking 'scope, proper use of microscope on and off and clean up procedures, use of Zeiss software, Zen.

Lab Mission/Purpose:

The Au lab is a developmental neurobiology lab that focuses on the inhibitory neurons of the neocortex: cortical interneurons. We are interested in the developmental process of cortical circuit assembly and how defects in this process may lead to neuropsychiatric illness. We use a broad array of approaches to address this question including, stem cell directed differentiation, in utero transplantation, primary culture, mouse genetics and image-based machine learning.

More information can be found: https://www.pathology.columbia.edu/research-labs/au-lab

Minimum Education Requirements:

Bachelor's degree in specific research field and 4 years experience preferred.

Minimum Skills Required:

Prior experience working in a wet bench lab and prior lab management responsibilities.

Preferred Qualifications:

Prior wetbench experience. Prior mouse husbandry experience a plus.

Columbia University is an Equal Opportunity Employer / Disability / Veteran

Pay Transparency Disclosure

The salary of the finalist selected for this role will be set based on a variety of factors, including but not limited to departmental budgets, qualifications, experience, education, licenses, specialty, and training. The above hiring range represents the University's good faith and reasonable estimate of the range of possible compensation at the time of posting.

Equal Opportunity Employer / Disability / Veteran

Columbia University is committed to the hiring of qualified local residents.

Minimum Salary: 31200.00 Maximum Salary: 31200.00 Salary Unit: Yearly