Campus Pride Jobs

Job ID 12532 Location Case Main Campus Full/Part Time Full-Time Regular/Temporary Regular Job Description Position Objective Working under limited supervision, the Research Assistant 3 will coordinate research activities on projects related to cardiac contractile function. The research assistant will work closely with other lab members and focus on research involving focus on research involving characterization of in vivo cardiac function utilizing ultrasound echocardiography. The research assistant will perform a wide array of cellular and molecular biology experiments using gel electrophoresis, western blots, bacterial transformation and culture, and other in vitro techniques. This position will utilize various molecular and in vivo methods to bridge the gap of understanding between molecular observations and cardiac function. This position will work with animals. Essential Functions Work closely with the principal investigator to coordinate research activities that characterize the roles of myofilament contractile proteins in the regulation of cardiac function using ultrasound. Tasks include but are not limited to, evaluating the efficacy of small molecules and gene therapy by systemic delivery in reversing systolic and diastolic dysfunction in genetic and acquired heart failure models. (40%) Collect, quantify and analyze data from general molecular biology experiments, including expression levels of cardiac proteins by various gel electrophoresis techniques, western blotting, and immunohistochemistry. (20%) Carry out general molecular biology, including design and implement cloning strategies, PCR, mutagenesis, gel electrophoresis, gel purification, plasmid preparation, bacterial transformation, protein biochemistry, bacterial culture, western blotting, and expression and purification of recombinant proteins. (10%) Carry out complex research assignments to characterize expression and localization of contractile proteins in the heart using ex vivo and in vivo fluorescent imaging techniques. Devise new protocols for in vivo gene delivery and techniques to achieve high exogenous protein expression in the heat of mice using a high degree of skill and training. (10%) Evaluate adequacy of biochemical techniques, study and test new procedures, analyze data, maintain lab records, provide feedback on experimental design, prepare and present research results in lab meetings. (10%) Assist in manuscript preparation and editing. May co-author research projects. (6%) NonEssential Functions May supervise students and technicians. (2%) Maintain equipment, prepare buffer/media/reagents, and dispose biohazardous waste. Oversee inventory, standard operating procedures, and monitor laboratory budget. (1%) Perform other duties as assigned. (1%) CONTACTS Department: Weekly contact with investigators to discuss research, research education, direction, goals and time management. Frequent contact with laboratory employees in collaboration in common research project. University: Occasional interaction with other faculty in the department and the university to provide updates on common collaborative research projects. Utilize core university facilities for experimentation and maintain strong relationship with core mangers for research collaborations. External: Occasional communication with research collaborators from other institutions. Students: Occasional contact with students to assist with student research projects. Supervisory Responsibility May supervise students and technicians. QUALIFICATIONS Experience: 3 to 5 years of related experience required. Experience working with mouse models of heart failure, molecular biology, protein biochemistry, and virology preferred. Education: Bachelor's degree in physiology, biomedical engineering, molecular biology, biochemistry,